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Acta
Biomaterialia
Volume
3, Issue 4, July 2007, Pages 457-462

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doi:10.1016/j.actbio.2006.12.006
Copyright © 2007 Acta Materialia Inc.
Published by Elsevier Ltd.

Brief communication

Cell adhesion and mechanical properties of a flexible scaffold for cardiac
tissue engineering

L.A. Hidalgo-Bastidaa,
J.J.A. Barrya,
N.M. Everittb,
F.R.A.J. Rosea,
L.D. Butterya,
I.P. Hallc,
W.C. Claycombd
and K.M. Shakesheffa,
,

aDivision of Drug Delivery and Tissue
Engineering, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD,
UK

bBioengineering, School of Mechanical, Materials
and Manufacturing Engineering, University of Nottingham, Nottingham NG7 2RD,
UK

cDivision of Therapeutics and Molecular Medicine,
School of Medical and Surgical Sciences, University Hospital Nottingham,
Nottingham NG7 2UH, UK

dBiochemistry and Molecular Biology, School of
Medicine at New Orleans, Louisiana State University, Baton Rouge, LA,
USA

Received 21 March 2006;

revised 7 December 2006;

accepted 12 December 2006.

Available online 26 February 2007.

This article is not included in your organization's
subscription. However, you may be able to access
this article under your organization's agreement with Elsevier.

Abstract

Cardiac tissue engineering is focused on obtaining functional cardiomyocyte
constructs to provide an alternative to cellular cardiomyoplasty. Mechanical
stimuli have been shown to stimulate protein expression and the differentiation
of mammalian cells from contractile tissues. Our aim was to obtain a flexible
scaffold which could be used to apply mechanical forces during tissue
regeneration. Poly(1,8-octanediol-co-citric acid) (POC) is an elastomer that can
be processed into scaffolds for tissue engineering. We investigated the effect
of modifying the porosity on the mechanical properties of the POC scaffolds. In
addition, the effects of the storage method and strain rate on material
integrity were assessed. The maximum elongation of POC porous films varied from
60% to 160% of their original length. A decrease in the porosity caused a rise
in this elastic modulus. The attachment of HL-1 cardiomyocytes to POC was
assessed on films coated with fibronectin, collagen and laminin. These
extracellular matrix proteins promoted cell adhesion in a protein-type- and
concentration-dependent manner. Therefore, POC scaffolds can be optimised to
meet the mechanical and biological parameters needed for cardiac culture. This
porous material has the potential to be used for cardiac tissue engineering as
well as for other soft tissue applications.

Keywords: Scaffold; Poly(1,8-octanediol-co-citric acid)
(POC); Elastomer; Cardiomyocyte; Tissue engineering

Article Outline

1. Introduction

2. Experimental

2.1. Poly(1,8-octanediol-co-citric
acid) synthesis

2.2. Fabrication
and modification of scaffolds

2.3. Scaffold
characterisation

2.4. Cell
culture of HL-1 cells

2.5. Cell
seeding on polymer films

3. Results

3.1. HL-1
adhesion to POC films

3.2. Characteristics
of POC processed scaffolds

4. Discussion

5. Conclusions

Acknowledgements

References

Fig. 1. (a) Total area coverage of HL-1 cells after 18 h of culture
on POC films coated with fibronectin, collagen or laminin
(n = 3, error bars represents +SD); (b) porous structure of a
POC scaffold with inter-connected pores as shown in SEM of the surface and in
(c) microcomputed tomography scan.

View Within Article

Fig. 2. Young’s modulus of POC scaffolds, stored at (a) −20 °C and
(b) at room temperature, tested at 18, 180, 300 and
500 mm min−1. Maximum elongation at break of POC scaffolds,
stored at (c) −20 °C and (d) at room temperature, tested at 18, 180, 300
and 500 mm min−1 (n = 3, error bars
represents +SD).

View Within Article

Acta
Biomaterialia
Volume
3, Issue 4, July 2007, Pages 457-462

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